|
|
|
Registros recuperados: 16 | |
|
| |
|
|
Fileti,Ana Maria Frattini; Fischer,Gilvan Anderson; Santana,José Carlos Curvelo; Tambourgi,Elias Basile. |
The main aim of this study was to optimize the conditions for bromelain extraction by reversed micelles from pineapple juice (Ananas comosus). The purification was carried out in batch extraction and a micro-column with pulsed caps for continuous extraction. The cationic micellar solution was made of BDBAC as a surfactant, isooctane as a solvent and hexanol as a co-solvent. For the batch process, a purification factor of 3 times at the best values of surfactant agent, co-solvent and salt concentrations, pH of the back and forward extractions were, 100 mM, 10% v/v, 1 M, 3.5 and 8, respectively. For the continuous operation, independent variables optimal point was determined: ratio between light phase flow rate and total flow rate equal to 0.67 and 1 second... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Reversed micelles; Liquid-liquid extraction; Bromelain; Pineapple; Optimization; Surface response. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500021 |
| |
|
| |
|
|
Leite,Nadjma Souza; Lima,Aloísio André Bomfim de; Santana,José Carlos Curvelo; Lopes,Francisco Luiz Gumes; Lédo,Ana da Silva; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de. |
This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymatic activity; Bromelain; Pérola pineapple; Imperial pineapple; Production in vitro. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500002 |
| |
|
| |
|
| |
|
|
Coêlho,Diego de Freitas; Tundisi,Louise Lacalendola; Cerqueira,Keilla Santos; Rodrigues,Jacqueline Rego da Silva; Mazzola,Priscila Gava; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de. |
Abstract Microalgae are aquatic unicellular microorganisms that can be found both in freshwater and marine systems; are capable of photosynthesis; and can grow as individual cells or associated in chains or small colonies. Microalgae cultivation has gained large momentum among researchers in the past decades due to their ability to produce value metabolites, remarkable photosynthetic efficiency, and versatile nature. The wide technological potential, and thus increasing amount of scattered knowledge, may become the very first barrier that a post graduating student, or any non-specialist reader, will face when introduced to the subject. In this review paper, we access the core aspects of microalgae technology, covering their main characteristics, and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microalgae; Microalgae Selection and Isolation; Green Products; Microalgae Cultivation; Bioactive Compounds. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000101000 |
| |
|
| |
|
|
Moreira,Keila Aparecida; Cavalcanti,Maria Taciana Holanda; Duarte,Helena Simões; Tambourgi,Elias Basile; Melo,Eduardo Henrique Magalhães de; Silva,Valdinete Lins; Porto,Ana Lúcia Figueiredo; Lima Filho,José Luiz de. |
The partial characterization of extracellular proteases from Streptomyces clavuligerus NRRL 3585 and 644 mutant was investigated. The enzyme production was carried out in batch fermentation using soy bean filtrate as nitrogen source. Maximum activity was obtained after 96h of fermentation with an initial pH of 7.0. The enzyme was partially purified by ammonium sulphate precipitation. Enzymes from the two strains retained 37% of their initial activities at pH 8.0 after 2 h incubation at 25ºC. Enzyme half-life at pH 8.0 and 60ºC was 40.30 and 53.32 min, respectively for both strains (partially purified extract). The optimum pH was obtained at pH 7.0-8.0 and 8.4 for enzymes produced for 3585 and 644 strains (crude extract), respectively, and 8.4 and 8.0 for... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptomyces clavuligerus; Extracellular proteases; Thermal stability; PH. |
Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300010 |
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
Registros recuperados: 16 | |
|
|
|